Paul Müller

Optical Diffraction Tomography for Single Cells

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Kurzfassung in Englisch

Analyzing the structure of a single cell based on its refractive index (RI) distribution is a common and valued approach, because it does not require any artificial markers. The RI is an inherent structural marker that can be quantified in three dimensions with optical diffraction tomography (ODT), an inverse scattering technique. This work reviews the theory of ODT and its implementation with an emphasis on single-cell analysis, identifying the Rytov approximation as the most efficient descriptor for light propagation. The accuracy of the reconstruction method is verified with in silico data and imaging artifacts associated with the inverse scattering approach are addressed. Furthermore, an experimental ODT setup is presented that consists of a bright-field microscope, a phase-imaging camera, and an optical trap combined with a microfluidic chip. A novel image analysis pipeline is proposed that addresses image corrections and frame alignment of the recorded data prior to the RI reconstruction. In addition, for a rotational axis that is tilted with respect to the image plane, an improved reconstruction algorithm is introduced and applied to single, suspended cells in vitro, achieving sub-cellular resolution.

weitere Metadaten

Tomografie, Beugung, Brechungsindex, Phasenmikroskopie
tomography, diffraction, refractive index, phase microscopy
SWD SchlagworteTomografie
DDC Klassifikation570
RVK KlassifikationWE 2100
HochschuleTechnische Universität Dresden
FakultätFakultät Mathematik und Naturwissenschaften
ProfessurProfessur für Zelluläre Maschinen
BetreuerProf. Dr. Jochen Guck
GutachterProf. Dr. Jochen Guck
Dr. Kevin Chalut
Tag d. Einreichung (bei der Fakultät)14.03.2016
Tag d. Verteidigung / Kolloquiums / Prüfung22.04.2016
Veröffentlichungsdatum (online)09.05.2016
persistente URNurn:nbn:de:bsz:14-qucosa-202261

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