Britta Koch

Scaffold dimensionality and confinement determine single cell morphology and migration

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Kurzfassung in Englisch

This thesis describes a highly interdisciplinary approach to discern the differing impact of scaffold dimensionality and physical space restrictions on the behavior of single cells. Rolled-up nanotechnology is employed to fabricate three-dimensional (3D) SiO/SiO2 microtube geometries of varied diameter, that after a biofunctionalization step are shown to support the growth of U2OS and six different types of stem cells. Cell confinement quantifiable through the given microtube diameter is tolerated by U2OS cells through a remarkable elongation of the cell body and nucleus down to a certain threshold, while the integrity of the DNA is maintained.

This confinement for NSPCs also leads to the approaching of the in vivo morphology, underlining the space-restrictive property of live tissue. The dimensionality of the cell culture scaffold however is identified as the major determiner of NSPC migration characteristics and leads to a morphologically distinct mesenchymal to amoeboid migration mode transition. The 3D microtube migration is characterized by exclusively filopodia protrusion formation, a higher dependence on actin polymerization and adopts aspects of in vivo-reported saltatory movement. The reported findings contribute to the determination of biomaterial scaffold design principles and advance our current understanding of how physical properties of the extracellular environment affect cell migration characteristics.

weitere Metadaten

Schlagwörter
(Deutsch)
Zellmigration, neuronale Stammzellen, Zellkulturgerüst, Microröhrchen
Schlagwörter
(Englisch)
cell migration, neural stem cells, cell culture scaffold, microtubes
DDC Klassifikation570
RVK KlassifikationWE 2300
Institution(en) 
HochschuleTechnische Universität Dresden
FakultätFakultät Mathematik und Naturwissenschaften
ProfessurProfessur für Zelluläre Maschinen
BetreuerProf. Dr. Jochen Guck
GutachterProf. Dr. Jochen Guck
Prof. Dr. Oliver G. Schmidt
DokumententypDissertation
SpracheEnglisch
Tag d. Einreichung (bei der Fakultät)18.09.2015
Tag d. Verteidigung / Kolloquiums / Prüfung15.01.2016
Veröffentlichungsdatum (online)18.01.2016
persistente URNurn:nbn:de:bsz:14-qucosa-194717

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