Mohd Noriznan Mokhtar

Biocatalytic Production, Preparation and Characterization of Large-ring Cyclodextrins

Dokumente und Dateien

Hinweis

Bitte nutzen Sie beim Zitieren immer folgende Url:

http://nbn-resolving.de/urn:nbn:de:bsz:ch1-200900431

Kurzfassung in Englisch

Cyclodextrins (CD) are cyclic oligosaccharides composed of six to more than sixty
glucose units. Large-ring cyclodextrins (LR-CD) are novel CD comprised of more than eight
glucose units with cavity structures and sizes different from that of commercially available
CD<sub>6</sub> – CD<sub>8</sub>. LR-CD may offer unique molecular recognition properties and can be produced
biocatalytically from starch using cyclodextrin glucanotransferase (CGTase, E.C. 2.4.1.19) in
a short reaction time. LR-CD were isolated from glucose, CD<sub>6</sub> – CD<sub>8</sub> and other compounds by
complexation of CD<sub>6</sub> – CD<sub>8</sub> as well as precipitation techniques. The yield of LR-CD (degree
of polymerization from 9 to 21) was optimized using central composite design. Addition of
polar organic solvents to the synthesis resulted in higher yields of LR-CD. LR-CD composed
of 9 to 21 glucose units were successfully separated using reversed-phase of ODS-AQ
chromatography and normal-phase of polyamine II chromatography. Maintaining optimized
reaction conditions aided in a high yield of CD<sub>9</sub>; it could be separated with reasonable yield
using a single step of polyamine II chromatography. A co-grinding method helped to obtain
higher solubilization levels of glibenclamide, vitamin A acetate and vitamin D<sub>3</sub> in CD<sub>13</sub>, CD<sub>10</sub>
and CD<sub>11</sub>, respectively when compared to other CD. Vitamin K<sub>1</sub> was solubilized in distilled
water with CD<sub>6</sub> – CD<sub>13</sub> using a co-precipitation method. When compared with other CD, CD<sub>9</sub>
was seen to be the best solubilizer. The analysis of complexes using ESI MS showed
spironolactone and glibenclamide complexed with CD<sub>9</sub> and CD<sub>13</sub>, respectively.

weitere Metadaten

Schlagwörter
Large-ring cyclodextrins (LR-CD)
Schlagwörter
central composite design
Schlagwörter
co-grinding
Schlagwörter
co-precipitation
Schlagwörter
cyclodextrin glucanotransferase (CGTase)
Schlagwörter
host-guest complexation
Schlagwörter
molecular recognition
Schlagwörter
supramolecular chemistry
SWD SchlagworteBacillus macerans
SWD SchlagworteMolekulare Erkennung
DDC Klassifikation570
Institution(en) 
HochschuleTU Chemnitz
FakultätFakultät für Maschinenbau
BetreuerProf. Dr. Wolfgang Zimmermann
GutachterProf. Dr. Wolfgang Zimmermann
Prof. Dr.-Ing. Bernd Platzer
Prof. Dr. Norbert Sträter
DokumententypDissertation
SpracheEnglisch
Tag d. Einreichung (bei der Fakultät)06.10.2008
Tag d. Verteidigung / Kolloquiums / Prüfung26.01.2009
Veröffentlichungsdatum (online)04.03.2009
persistente URNurn:nbn:de:bsz:ch1-200900431

Hinweis zum Urheberrecht

Diese Website ist eine Installation von Qucosa - Quality Content of Saxony!
Sächsische Landesbibliothek Staats- und Universitätsbibliothek Dresden